te‐2000e point scanning confocal microscope Search Results


inverted point laser scanning confocal microscope zeiss lsm980 axio observer z1 7  (Carl Zeiss)
99
Carl Zeiss inverted point laser scanning confocal microscope zeiss lsm980 axio observer z1 7
Inverted Point Laser Scanning Confocal Microscope Zeiss Lsm980 Axio Observer Z1 7, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inverted point scanning confocal microscope zeiss axio observer.z1  (Carl Zeiss)
90
Carl Zeiss inverted point scanning confocal microscope zeiss axio observer.z1
Inverted Point Scanning Confocal Microscope Zeiss Axio Observer.Z1, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lsm700 single-point, laser scanning confocal microscope  (Carl Zeiss)
90
Carl Zeiss lsm700 single-point, laser scanning confocal microscope
a Percentages of inflammatory M1- and immunosuppressive M2-macrophages and M-MDSCs arising from primary murine macrophages. b Percentages of inflammatory, TNF-α + M1, and IL-6 + inflammatory macrophages (M1-like), and c CD206 + CD124 + and IL-10 + immunosuppressive macrophages (M2-like) arising from primary human macrophages. Data were collected after 24 h exposures at MOI of 20:1 as determined by flow-cytometry using gating schemes shown in Fig. – . Data are presented as mean values ± S.E.M. ( n = 4 independent biological replicate experiments). d Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. Data are represented as mean values ± S.E.M ( n = 3 independent biological replicate experiments). e Autophagy induction and f quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an <t>LSM700</t> confocal microscope and Fiji software processing. Quantification was measured by mean fluorescence intensity. Data represented for all confocal microscopy studies (phagocytosis, LC3B-BCG colocalization and p62-BCG colocalization) as mean values ± S.E.M. ( n = 3 independent biological replicate experiments). All statistical analyses done using two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Figs. and .
Lsm700 Single Point, Laser Scanning Confocal Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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fv000 point-scanning confocal microscope  (Evident Corporation)
90
Evident Corporation fv000 point-scanning confocal microscope
a Percentages of inflammatory M1- and immunosuppressive M2-macrophages and M-MDSCs arising from primary murine macrophages. b Percentages of inflammatory, TNF-α + M1, and IL-6 + inflammatory macrophages (M1-like), and c CD206 + CD124 + and IL-10 + immunosuppressive macrophages (M2-like) arising from primary human macrophages. Data were collected after 24 h exposures at MOI of 20:1 as determined by flow-cytometry using gating schemes shown in Fig. – . Data are presented as mean values ± S.E.M. ( n = 4 independent biological replicate experiments). d Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. Data are represented as mean values ± S.E.M ( n = 3 independent biological replicate experiments). e Autophagy induction and f quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an <t>LSM700</t> confocal microscope and Fiji software processing. Quantification was measured by mean fluorescence intensity. Data represented for all confocal microscopy studies (phagocytosis, LC3B-BCG colocalization and p62-BCG colocalization) as mean values ± S.E.M. ( n = 3 independent biological replicate experiments). All statistical analyses done using two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Figs. and .
Fv000 Point Scanning Confocal Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inverse point scanning confocal microscope zeiss lsm 710 airyscan  (Carl Zeiss)
90
Carl Zeiss inverse point scanning confocal microscope zeiss lsm 710 airyscan
a Percentages of inflammatory M1- and immunosuppressive M2-macrophages and M-MDSCs arising from primary murine macrophages. b Percentages of inflammatory, TNF-α + M1, and IL-6 + inflammatory macrophages (M1-like), and c CD206 + CD124 + and IL-10 + immunosuppressive macrophages (M2-like) arising from primary human macrophages. Data were collected after 24 h exposures at MOI of 20:1 as determined by flow-cytometry using gating schemes shown in Fig. – . Data are presented as mean values ± S.E.M. ( n = 4 independent biological replicate experiments). d Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. Data are represented as mean values ± S.E.M ( n = 3 independent biological replicate experiments). e Autophagy induction and f quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an <t>LSM700</t> confocal microscope and Fiji software processing. Quantification was measured by mean fluorescence intensity. Data represented for all confocal microscopy studies (phagocytosis, LC3B-BCG colocalization and p62-BCG colocalization) as mean values ± S.E.M. ( n = 3 independent biological replicate experiments). All statistical analyses done using two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Figs. and .
Inverse Point Scanning Confocal Microscope Zeiss Lsm 710 Airyscan, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inverse point scanning confocal microscope zeiss lsm 710 airyscan - by Bioz Stars, 2026-03
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confocal point-scanning microscope using both z-stack and scan tile zeiss lsm 800 with airyscan  (Carl Zeiss)
90
Carl Zeiss confocal point-scanning microscope using both z-stack and scan tile zeiss lsm 800 with airyscan
a Percentages of inflammatory M1- and immunosuppressive M2-macrophages and M-MDSCs arising from primary murine macrophages. b Percentages of inflammatory, TNF-α + M1, and IL-6 + inflammatory macrophages (M1-like), and c CD206 + CD124 + and IL-10 + immunosuppressive macrophages (M2-like) arising from primary human macrophages. Data were collected after 24 h exposures at MOI of 20:1 as determined by flow-cytometry using gating schemes shown in Fig. – . Data are presented as mean values ± S.E.M. ( n = 4 independent biological replicate experiments). d Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. Data are represented as mean values ± S.E.M ( n = 3 independent biological replicate experiments). e Autophagy induction and f quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an <t>LSM700</t> confocal microscope and Fiji software processing. Quantification was measured by mean fluorescence intensity. Data represented for all confocal microscopy studies (phagocytosis, LC3B-BCG colocalization and p62-BCG colocalization) as mean values ± S.E.M. ( n = 3 independent biological replicate experiments). All statistical analyses done using two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Figs. and .
Confocal Point Scanning Microscope Using Both Z Stack And Scan Tile Zeiss Lsm 800 With Airyscan, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
confocal point-scanning microscope using both z-stack and scan tile zeiss lsm 800 with airyscan - by Bioz Stars, 2026-03
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point scanning confocal microscopy  (Carl Zeiss)
90
Carl Zeiss point scanning confocal microscopy
a Percentages of inflammatory M1- and immunosuppressive M2-macrophages and M-MDSCs arising from primary murine macrophages. b Percentages of inflammatory, TNF-α + M1, and IL-6 + inflammatory macrophages (M1-like), and c CD206 + CD124 + and IL-10 + immunosuppressive macrophages (M2-like) arising from primary human macrophages. Data were collected after 24 h exposures at MOI of 20:1 as determined by flow-cytometry using gating schemes shown in Fig. – . Data are presented as mean values ± S.E.M. ( n = 4 independent biological replicate experiments). d Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. Data are represented as mean values ± S.E.M ( n = 3 independent biological replicate experiments). e Autophagy induction and f quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an <t>LSM700</t> confocal microscope and Fiji software processing. Quantification was measured by mean fluorescence intensity. Data represented for all confocal microscopy studies (phagocytosis, LC3B-BCG colocalization and p62-BCG colocalization) as mean values ± S.E.M. ( n = 3 independent biological replicate experiments). All statistical analyses done using two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Figs. and .
Point Scanning Confocal Microscopy, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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point scanning confocal microscopy - by Bioz Stars, 2026-03
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700-point scanning confocal microscope with a 63 lens with oil  (Carl Zeiss)
90
Carl Zeiss 700-point scanning confocal microscope with a 63 lens with oil
a Percentages of inflammatory M1- and immunosuppressive M2-macrophages and M-MDSCs arising from primary murine macrophages. b Percentages of inflammatory, TNF-α + M1, and IL-6 + inflammatory macrophages (M1-like), and c CD206 + CD124 + and IL-10 + immunosuppressive macrophages (M2-like) arising from primary human macrophages. Data were collected after 24 h exposures at MOI of 20:1 as determined by flow-cytometry using gating schemes shown in Fig. – . Data are presented as mean values ± S.E.M. ( n = 4 independent biological replicate experiments). d Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. Data are represented as mean values ± S.E.M ( n = 3 independent biological replicate experiments). e Autophagy induction and f quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an <t>LSM700</t> confocal microscope and Fiji software processing. Quantification was measured by mean fluorescence intensity. Data represented for all confocal microscopy studies (phagocytosis, LC3B-BCG colocalization and p62-BCG colocalization) as mean values ± S.E.M. ( n = 3 independent biological replicate experiments). All statistical analyses done using two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Figs. and .
700 Point Scanning Confocal Microscope With A 63 Lens With Oil, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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700-point scanning confocal microscope with a 63 lens with oil - by Bioz Stars, 2026-03
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micrometer standard grid plate  (Carl Zeiss)
90
Carl Zeiss micrometer standard grid plate
a Percentages of inflammatory M1- and immunosuppressive M2-macrophages and M-MDSCs arising from primary murine macrophages. b Percentages of inflammatory, TNF-α + M1, and IL-6 + inflammatory macrophages (M1-like), and c CD206 + CD124 + and IL-10 + immunosuppressive macrophages (M2-like) arising from primary human macrophages. Data were collected after 24 h exposures at MOI of 20:1 as determined by flow-cytometry using gating schemes shown in Fig. – . Data are presented as mean values ± S.E.M. ( n = 4 independent biological replicate experiments). d Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. Data are represented as mean values ± S.E.M ( n = 3 independent biological replicate experiments). e Autophagy induction and f quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an <t>LSM700</t> confocal microscope and Fiji software processing. Quantification was measured by mean fluorescence intensity. Data represented for all confocal microscopy studies (phagocytosis, LC3B-BCG colocalization and p62-BCG colocalization) as mean values ± S.E.M. ( n = 3 independent biological replicate experiments). All statistical analyses done using two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Figs. and .
Micrometer Standard Grid Plate, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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micrometer standard grid plate - by Bioz Stars, 2026-03
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point-scanning confocal microscope on an upright stand  (Carl Zeiss)
90
Carl Zeiss point-scanning confocal microscope on an upright stand
a Percentages of inflammatory M1- and immunosuppressive M2-macrophages and M-MDSCs arising from primary murine macrophages. b Percentages of inflammatory, TNF-α + M1, and IL-6 + inflammatory macrophages (M1-like), and c CD206 + CD124 + and IL-10 + immunosuppressive macrophages (M2-like) arising from primary human macrophages. Data were collected after 24 h exposures at MOI of 20:1 as determined by flow-cytometry using gating schemes shown in Fig. – . Data are presented as mean values ± S.E.M. ( n = 4 independent biological replicate experiments). d Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. Data are represented as mean values ± S.E.M ( n = 3 independent biological replicate experiments). e Autophagy induction and f quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an <t>LSM700</t> confocal microscope and Fiji software processing. Quantification was measured by mean fluorescence intensity. Data represented for all confocal microscopy studies (phagocytosis, LC3B-BCG colocalization and p62-BCG colocalization) as mean values ± S.E.M. ( n = 3 independent biological replicate experiments). All statistical analyses done using two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Figs. and .
Point Scanning Confocal Microscope On An Upright Stand, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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point-scanning confocal microscope on an upright stand - by Bioz Stars, 2026-03
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inverse point scanning confocal zeiss lsm980 microscope  (Carl Zeiss)
90
Carl Zeiss inverse point scanning confocal zeiss lsm980 microscope
a Percentages of inflammatory M1- and immunosuppressive M2-macrophages and M-MDSCs arising from primary murine macrophages. b Percentages of inflammatory, TNF-α + M1, and IL-6 + inflammatory macrophages (M1-like), and c CD206 + CD124 + and IL-10 + immunosuppressive macrophages (M2-like) arising from primary human macrophages. Data were collected after 24 h exposures at MOI of 20:1 as determined by flow-cytometry using gating schemes shown in Fig. – . Data are presented as mean values ± S.E.M. ( n = 4 independent biological replicate experiments). d Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. Data are represented as mean values ± S.E.M ( n = 3 independent biological replicate experiments). e Autophagy induction and f quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an <t>LSM700</t> confocal microscope and Fiji software processing. Quantification was measured by mean fluorescence intensity. Data represented for all confocal microscopy studies (phagocytosis, LC3B-BCG colocalization and p62-BCG colocalization) as mean values ± S.E.M. ( n = 3 independent biological replicate experiments). All statistical analyses done using two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Figs. and .
Inverse Point Scanning Confocal Zeiss Lsm980 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inverse point scanning confocal zeiss lsm980 microscope - by Bioz Stars, 2026-03
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lsm10 point scanning confocal microscope  (Carl Zeiss)
90
Carl Zeiss lsm10 point scanning confocal microscope
a Percentages of inflammatory M1- and immunosuppressive M2-macrophages and M-MDSCs arising from primary murine macrophages. b Percentages of inflammatory, TNF-α + M1, and IL-6 + inflammatory macrophages (M1-like), and c CD206 + CD124 + and IL-10 + immunosuppressive macrophages (M2-like) arising from primary human macrophages. Data were collected after 24 h exposures at MOI of 20:1 as determined by flow-cytometry using gating schemes shown in Fig. – . Data are presented as mean values ± S.E.M. ( n = 4 independent biological replicate experiments). d Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. Data are represented as mean values ± S.E.M ( n = 3 independent biological replicate experiments). e Autophagy induction and f quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an <t>LSM700</t> confocal microscope and Fiji software processing. Quantification was measured by mean fluorescence intensity. Data represented for all confocal microscopy studies (phagocytosis, LC3B-BCG colocalization and p62-BCG colocalization) as mean values ± S.E.M. ( n = 3 independent biological replicate experiments). All statistical analyses done using two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Figs. and .
Lsm10 Point Scanning Confocal Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a Percentages of inflammatory M1- and immunosuppressive M2-macrophages and M-MDSCs arising from primary murine macrophages. b Percentages of inflammatory, TNF-α + M1, and IL-6 + inflammatory macrophages (M1-like), and c CD206 + CD124 + and IL-10 + immunosuppressive macrophages (M2-like) arising from primary human macrophages. Data were collected after 24 h exposures at MOI of 20:1 as determined by flow-cytometry using gating schemes shown in Fig. – . Data are presented as mean values ± S.E.M. ( n = 4 independent biological replicate experiments). d Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. Data are represented as mean values ± S.E.M ( n = 3 independent biological replicate experiments). e Autophagy induction and f quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was measured by mean fluorescence intensity. Data represented for all confocal microscopy studies (phagocytosis, LC3B-BCG colocalization and p62-BCG colocalization) as mean values ± S.E.M. ( n = 3 independent biological replicate experiments). All statistical analyses done using two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Figs. and .

Journal: Nature Communications

Article Title: Re-engineered BCG overexpressing cyclic di-AMP augments trained immunity and exhibits improved efficacy against bladder cancer

doi: 10.1038/s41467-022-28509-z

Figure Lengend Snippet: a Percentages of inflammatory M1- and immunosuppressive M2-macrophages and M-MDSCs arising from primary murine macrophages. b Percentages of inflammatory, TNF-α + M1, and IL-6 + inflammatory macrophages (M1-like), and c CD206 + CD124 + and IL-10 + immunosuppressive macrophages (M2-like) arising from primary human macrophages. Data were collected after 24 h exposures at MOI of 20:1 as determined by flow-cytometry using gating schemes shown in Fig. – . Data are presented as mean values ± S.E.M. ( n = 4 independent biological replicate experiments). d Phagocytic activity in human primary macrophages in representative confocal photomicrographs showing intracellular uptake of FITC-labeled IgG-opsonized latex beads (green) with nuclei stained blue. Data are represented as mean values ± S.E.M ( n = 3 independent biological replicate experiments). e Autophagy induction and f quantification by BCG-LC3B colocalization in primary murine macrophages shown by representative confocal photomicrographs. Autophagy was measured by LC3B puncta or g . p62 colocalization with BCG appearing in yellow. FITC-labeled BCG strains are stained green, LC3B or p62 autophagic puncta (red), and nuclei blue. h Quantification of BCG-p62 colocalization. Cells were fixed using 4% paraformaldehyde 6 h after infection (MOI 10:1), and images obtained with an LSM700 confocal microscope and Fiji software processing. Quantification was measured by mean fluorescence intensity. Data represented for all confocal microscopy studies (phagocytosis, LC3B-BCG colocalization and p62-BCG colocalization) as mean values ± S.E.M. ( n = 3 independent biological replicate experiments). All statistical analyses done using two-tailed Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001). Data shown are for BCG-Tice; similar findings were observed for BCG-Pasteur as shown in Figs. and .

Article Snippet: Infection was carried out at 5:1 (HMDM versus BCG) ratio for 3 h followed by addition of IgG-FITC beads in warm RPMI 1640 media at 1: 400 dilutions for 3 h. Nuclear staining was carried out using Hoechst 33342 (Cat. 62249, Thermo Scientific) and cells were visualized for bead phagocytosis using Zeiss LSM700 single-point, laser scanning confocal microscope.

Techniques: Flow Cytometry, Activity Assay, Labeling, Staining, Infection, Microscopy, Software, Fluorescence, Confocal Microscopy, Two Tailed Test